生物燃气与环境工程中心是研究所核心组群之一，承担了中国科学院在生物天然气领域唯一的省部级研发平台-生物天然气山东省工程研究中心。主要从事农业、城市有机废弃物制备生物天然气技术研究、装备开发和产业化推广，同时拓展出了污水治理新材料、工业尾气及其他一碳气体制备微生物蛋白等研究方向。先后承担中国科学院重大突破项目“秸秆基百万立方生物天然气产业化系统”、中国科学院科技成果转移转化重点专项“分布式农业生物质沼气规模化生产与利用产业化”、中国科学院战略性先导科技专项A类课题“城镇化区域生物天然气综合利用与分布式供能工业化示范”及其他国家重点研发计划、973课题、863课题、重大装备研制等项目100余项。开发的生物天然气产业链技术与装备体系2016年即经院士领衔的专家组鉴定为技术水平“国内领先、国际先进”,中心的技术创新和产业化推广在业内产生了重要影响，最新开发的多级仿生厌氧发酵技术引领行业技术更新换代。

代表性项目：

代表性奖励：

代表性专利：

1. 一种湿法—干法联合的两级厌氧发酵产沼气工艺

2. 一种可在线维护或更换的搅拌器及其维护或更换方法

4. 一种常压水洗沼气脱碳净化的方法

5. 气体膜分离同步回收液态CO2的沼气脱碳工艺和装置

6. 一种开合臂式生物质固态定量送料装置

7. 一种用于MABR的快速启动定向包埋微生物膜及其应用

8. 一种太阳能电解水与厌氧好氧联用生物水处理系统

9. 一种兼性自养氢氧化菌、获取方法及应用

10. 一种由秸秆制备腐植酸的方法

代表性论文：

1. Dongxue Lu, Min Li, Erqi Nie, Rongbo Guo, Shanfei Fu. Microbial volatile organic compounds produced during the anaerobic digestion process can serve as potential indicators of microbial community stability. Water Research 277(2025) 123286.

2. Yu Zhang, Qingyu Yin, Dongxue Lu, Changxue Wang, Tingfeng Cheng,  and Shanfei Fu. Co-hydrothermal treatment of straw and zeolite to produce a slow-release fertilizer as a new strategy to promote biomass waste utilization and improve the properties of saline-alkali soil. J. Agric. Food Chem. 2025, 73, 18617−18631

3. Rui Li, Ming-yi Lu, Rong-bo Guo, Haoran Duan, Bing-jie Ni, Shan-fei Fu,Life cycle assessment of hydrogenotrophic denitrification in membrane aerated biofilm reactors for sustainable wastewater treatment,Water Research 267 (2024) 122529.

4. Hai-Ming Huang, Zhi-Xin Xue, Yu-Feng Jiang, Rui Li, Rong-Bo Guo, Xiao-Lei Fan , Shan-Fei Fu,New approach for raw biogas: Production of single cell protein by  sulfide-tolerant methane-oxidizing bacteria consortia,Chemical Engineering Journal 495 (2024) 153678

5. R. Li, Y. Jiang, J. Huang, K. Luo, X. Fan, R. Guo, T. Liu, Y. Zhang, S. Fu, Simultaneous biogas upgrading and single cell protein production using hydrogen oxidizing bacteria, Chemical Engineering Journal 490 (2024) 151576.

6. Mingyi Lu, Feng Zhao, Fan Qin, Fengyuan Zhang, Quan Feng, Rongbo Guo，Novel flocking materials as biocarriers in moving bed biofilm reactor for improving simultaneous nitrification and denitrification performance，Bioresource Technology 396 (2024) 13043

7. Rui Li, XiaoLei Fan, YuFeng Jiang, RuoNan Wang, RongBo Guo, Yifeng Zhang, ShanFei Fu. From anaerobic digestion to single cell protein synthesis: A promising route beyond biogas utilization. Water Research, 2023, 243, 120417.

8. Shanfei Fu, Shujuan Lian, Irini Angelidaki, Rongbo Guo. Micro-aeration: an attractive strategy to facilitate anaerobic digestion. Trends in Biotechnology, 2023, 41, 714-726.

9. Jun Lu, Zhiman Yang, Wanying Xu, Xiaoshuang Shi, Rongbo Guo. Enrichment of thermophilic and mesophilic microbial consortia for efficient degradation of corn stalk. Joural of Environmental Science, 2019(4):118-126.

10. Yang, D. W., Fan, X. L., Shi, X. S., Lian, S. J., Qiao, J. T., Guo, R. B..Metabolomics reveals stage-specific metabolic pathways of microbial communities in two-stage anaerobic fermentation of corn-stalkBiotechnology Letters,2014,36(7):1461-1468.